Metal and anion binding sites in lactoferrin and related proteins
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چکیده
The metal and anion binding sites of the protein lactoferrin (Lf) have been defined through crystallographic analyses of FegLf and Cu2Lf. In both cases each metal ion is 6-coordinate, with four protein ligands ( 2 Tyr, 1 Asp, 1 His) and the synergistic C032anion which binds as a bidentate ligand. The C032fits into a pocket between the metal and the N-terminus of an a-helix. Binding of other metals and anions can be understood in terms of the protein structure and a suggested mechanism for binding and release. Differences between the two sites in Lf can also be explained. suggest links between bacterial and mammalian binding proteins. Striking similarities between Lf and a S042binding protein
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تاریخ انتشار 2005